Identification & Characterization of Small Molecule RNA binders with Affinity Selection Mass Spectrometry (ASMS)

Time: 12:00 pm
day: Day One


  • PureHoney Tech has optimized a process for high throughput screening of large chemical libraries to identify small molecule binders of RNA targets using ASMS. As many as 100K test compounds can be screened in a little over a day from which hit lists can be generated. The only limitation on target size is solubility allowing for oligos as small as 20 bases and as large as thousands of bases to be screened
  • The binding affinity of hits can be determined by running dose-response experiments to generate binding curves. Alternately the binding affinity of up to 10 test compounds can be rapidly rank ordered by running the test compounds in pools against different concentrations of the RNA target
  • ASMS has been successfully used as a tool in multiple SAR and medicinal chemistry supporting drug development programs